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STAINING POTENTIAL OF BAPHIA NITIDA (CAMWOOD) DYE ON BACTERIA CELL AND MALARIA PARASITE

CHAPTER ONE

INTRODUCTION

1.0 BACKGROUND OF THE STUDY

Dyes are substances, which impact more or less permanent colour to other materials. They have in no small measure helped to reveal hidden histological and cytological facts. Owing to their importance they have become a focal point in scientific discoveries pertaining to tissues and cells. The use of dyes dates from centuries back. .According to Barker (1970), its origin must have arisen accidentally as a result of staining from berries or other plant parts, and therefore the deliberate use of these plants to produce colours probably followed soon after. Gerald (1979) stated that madder and indigo were first known in India from around 2500 BC while around 3000 BC dyeing has being an established craft in China.  Banerjee and Mukherjee (1981) mentioned some of these dyes as santalin and santarubin which are dyes contained in the camwood (Baphia nitida). Most natural dyes are of the mordant type which requires a fixing agent such as acetic acid or alum .

Nigeria and of course other countries depend so much on products manufactured by the developed countries, hence they are branded consumers countries. Nigeria is much dependent on developed countries for supply of hospital and laboratory consumables. Various Nigeria governments have been spending millions of naira and foreign currency on the purchase of dyes or biological stains from overseas. It is so appalling that no single biological stain used in laboratories is made in Nigeria, not even a foreign company in Nigeria

The tropical countries including Nigeria have greater diversity of dye producing plants. These include, Baphia nitida, among others.

Baphia nitida (camwood) is a small tree or shrubs up to 30ft high with glabrous branchlets, flowers usually 1-4 together, fragrant, white with a yellow center .The Ibos call it ‘Uhie, or ‘abosi’ and it is used for colouring palms and legs.

The availability of this plant locally and the much dependence on foreign dyes call for immediate research into the use of Nigerian plant extracts as biological stains in the ensuring work attempt was made to extract and use pigments from indigenous Nigeria plants as biological stains for parasites of medical importance.

1.1 AIM

To determine the staining potentials of the B.nitida.

1.2 OBJECTIVES

Because it is cheap and always available, it therefore can save cost and overdependence on foreign staining reagents(Giemsa and Lieshman).

It can also serve as an alternative for further scientific discovery.

—This article is not complete———–This article is not complete———— This article was extracted from a well articulated quality Project, Research Work/Material

Topic: Accessing the Staining Potential of Dye Extracted From Baphia Nitida (Camwood) on Bacteria Cell and Malaria Parasite

 

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Accessing the Staining Potential of Dye Extracted From Baphia Nitida (Camwood) on Bacteria Cell and Malaria Parasite

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